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HelpTest Code WGSEQ Gamma-Globin Full Gene Sequencing, Varies
Necessary Information
A complete patient history is strongly encouraged.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Whole blood
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD)
Specimen Volume: 4 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in the original tube. Do not aliquot.
Specimen Stability Information: Refrigerate 30 days(preferred)/Ambient 14 days
Specimen Type: Extracted DNA from whole blood
Container/Tube: 1.5 to 2 mL tube
Specimen Volume: Entire specimen
Collection Instructions: Label specimen as extracted DNA from blood and provide indication of volume and concentration of the DNA
Specimen Stability Information: Frozen (preferred)/Refrigerate/Ambient
Forms
1. New York Clients-Informed consent is required. Document on the request form or electronic order that a copy is on file. The following documents are available:
-Informed Consent for Genetic Testing (T576)
-Informed Consent for Genetic Testing-Spanish (T826)
2. Thalassemia/Hemoglobinopathy Patient Information (T358)
3. If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.
Useful For
An adjunct in the interpretation of hemoglobin electrophoresis results
Evaluation for suspected gamma variants or nondeletional hereditary persistence of fetal hemoglobin
Assessment of unstable gamma chain variants when other tests for causes of hemolysis are unrevealing
Special Instructions
Method Name
Polymerase Chain Reaction (PCR) Amplification/Sanger Sequence Analysis
Reporting Name
Gamma Globin Full Gene SequencingSpecimen Type
VariesSpecimen Minimum Volume
Blood: 1 mL; Extracted DNA: 50 mcL at 50 ng/mcL concentration
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | ||
Reject Due To
| Gross hemolysis | OK |
| Bone marrow Paraffin-embedded tissue Frozen tissue Paraffin-embedded bone marrow aspirate clot Methanol-acetic acid (MAA)-fixed pellets Moderately to severely clotted |
Reject |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Friday
Report Available
10 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
81479-Unlisted molecular
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| WGSEQ | Gamma Globin Full Gene Sequencing | 95795-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 46952 | Gamma Globin Gene Sequencing Result | 50397-9 |
| 46953 | Gamma Globin Interpretation | 59466-3 |