Test Code F_12 Coagulation Factor XII Activity Assay, Plasma
Reporting Name
Coag Factor XII Assay, PUseful For
Diagnosing deficiency of coagulation factor XII
Determining cause of prolonged activated partial thromboplastin time
Method Name
Optical Clot-Based
Performing Laboratory
Mayo Clinic Laboratories in RochesterSpecimen Type
Plasma Na CitOrdering Guidance
Coagulation testing is highly complex, often requiring the performance of multiple assays and correlation with clinical information. For that reason, consider ordering a Coagulation Consultation.
Necessary Information
If priority specimen, mark request form, give reason, and request a call-back.
Specimen Required
Specimen Type: Platelet-poor plasma
Patient Preparation: Patient must not be receiving Coumadin (warfarin) or heparin therapy.
Collection Container/Tube: Light-blue top (3.2% sodium citrate)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Specimen must be collected prior to factor replacement therapy
2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.
3. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.
4. Aliquot plasma into a plastic vial, leaving 0.25 mL in the bottom of centrifuged vial.
5. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or ideally, at or below -40° C.
Additional Information:
1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
2. Each coagulation assay requested should have its own vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Plasma Na Cit | Frozen | 14 days |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | Reject |
Gross icterus | Reject |
Special Instructions
Reference Values
Adults: 55-180%
Normal, full-term newborn infants or healthy premature infants may have decreased levels (≥15% to 20%), which may not reach adult levels for 180 or more days postnatal.*
*See Pediatric Hemostasis References section in Coagulation Guidelines for Specimen Handling and Processing.
Day(s) Performed
Monday through Saturday
CPT Code Information
85280
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
F_12 | Coag Factor XII Assay, P | 3232-6 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
F_12 | Coag Factor XII Assay, P | 3232-6 |
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.Report Available
1 to 3 daysForms
If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.