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Test Code FUNG Culture, Fungus

Additional Codes

YRMC Care - LAB240

Performing Laboratory

YRMC Microbiology

Day(s) Test Set Up

Monday through Sunday

Specimen Requirements

Submit only 1 of the following specimens:


Body Fluid

  • Container/Tube: Evacuated collection bottle or Screw-capped, sterile container(s)
  • Specimen Volume: 1 mL to 2 mL of body fluid

Nails (Finger or Toe)


  • Container/Tube: Screw-capped, sterile container(s)
  • Specimen Volume: 3 to 5 pieces of fingernails or toenails

Sputum, Expectorated


  • Container/Tube: Screw-capped, sterile container(s)
  • Specimen Volume: Early-Morning expectorated sputum (minimum volume: 5 mL) on at least 3 consecutive days.

Sputum, Induced


  • Container/Tube: Screw-capped, sterile container(s)
  • Specimen Volume: Early-Morning induced sputum (minimum volume: 5 mL) on at least 3 consecutive days.

Bronchial Wash or Bronchial Lavage


  • Container/Tube: Aspirate trap
  • Specimen Volume: Early-Morning induced sputum (minimum volume: 5 mL) on at least 3 consecutive days.

Tissue


  • Container/Tube: Screw-capped, sterile container(s) without fixative or preservative-Specimen in formalin is not acceptable
  • Specimen Volume: 1g of tissue, if possible

Specimen Transport Temperature

Ambient

Methodology

Conventional mycology culture methods
Includes KOH preparation

Reference Values

Negative For Fungus at 6 Weeks

Test Classification and CPT Coding

87102-Culture
87220-KOH Prep

Loinc Code

580-1

Specimen Collection Instructions

NOTE: SPECIMEN SOURCE IS REQUIRED ON ALL SPECIMEN TYPES
Body Fluid Collection Instructions:
1. Label container with patient’s name (first, last, and middle initial), medical record number (if appropriate), date of birth, date and time collection and collectors initials and/or ID number.


Nails (Finger or Toe) Collection Instructions:
1. Label container with patient’s name (first, last, and middle initial), medical record number (if appropriate), date of birth, date and time collection and collectors initials and/or ID number.


Sputum, Expectorated Collection Instructions:
1. Have patient remove dentures, if applicable.
2. Instruct patient to brush his/her teeth and/or rinse mouth well with water to minimize contaminating specimen with food particles, mouthwash, or oral drugs which may inhibit growth of mycobacteria.
3. Instruct patient to take a deep breath, hold it momentarily, then cough deeply and vigorously into container. Nasal secretions, saliva, or 24-hour collection is not acceptable.
4. Label container with patient’s name (first, last, and middle initial), medical record number (if appropriate), date of birth, date and time collection and collectors initials and/or ID number.


Sputum, Induced Collection Instructions:
1. Induce cough by inhalation of sterile, hypertonic saline, and collect expectorated material. Avoid sputum contamination with nebulizer reservoir water.
2. Label container with patient’s name (first, last, and middle initial), medical record number (if appropriate), date of birth, date and time of specimen collection and collectors initials and/or ID number.
3. Indicate if specimen is induced sputum, as these watery specimens resemble saliva and risk rejection as inadequate.


Bronchial Wash or Bronchial Lavage Collection Instructions:
1. Induce cough by inhalation of sterile, hypertonic saline, and collect expectorated material. Avoid sputum contamination with nebulizer reservoir water.
2. Label container with patient’s name (first, last, and middle initial), medical record number (if appropriate), date of birth, date and time of specimen collection and collectors initials and/or ID number.
3. Indicate if specimen is induced sputum, as these watery specimens resemble saliva and risk rejection as inadequate.


Tissue Collection Instructions:
1. Collect aseptically avoiding indigenous microbiota. Select caseous portion if available. Do not immerse in saline or other fluid or wrap in gauze.
2. Label container with patient’s name (first, last, and middle initial), medical record number, date of birth, date and time of collection and collector’s initials and or ID number.