Test Code CSAF Acid-Fast Bacillus (AFB) CSF Culture and Smear AFB Studies, (MTB) Mycobacterium Tuberculosis Culture, (TB) Tuberculosis
Additional Codes
AFB Culture and Stain- LAB877
Performing Laboratory
YRMC Microbiology
Day(s) Test Set Up
Monday through Friday
AFB smears are available Sunday/Saturday upon STAT request.
Specimen Requirements
All specimens must be submitted in a leak-proof container and sealed in a biohazard bag. Specimens that are received leaking or spilled will be rejected for testing.
Cerebrospinal Fluid Container/Tube:
- Sterile screw-capped collection container.
Specimen Volume:
- 1 mL (minimum volume)
Specimen Transport Temperature
Specimen must not be sent through the pneumatic tube system, must be hand carried to the laboratory.
Transport CSF specimens to the laboratory immediately. DO NOT REFRIGERATE.
For all other specimens, transport to the laboratory immediately. If delayed, refrigerate at 2 8°C.
Testing Algorithm
Test includes culture and smear.
Methodology
Culture, liquid medium and solid Lowenstein Jensen Medium
Flourescent Acid Fast Bacillus Stain
Reference Values
Negative for AFB at 6 weeks
Specimen Collection Instructions
1. Label container with patient’s name (first, last, and middle initial), medical record number (if appropriate), date of birth, date and time of collection and collector’s initials and/or ID number
2. Specimen source is required
Test Classification and CPT Coding
87116 – AFB Culture