Test Code BLC Culture, Blood
Additional Codes
YRMC Care - LAB462
Performing Laboratory
YRMC Microbiology
Day(s) Test Set Up
Sunday through Saturday, continuous monitoring for 5 days after culture is received in laboratory.
All recovered organisms are identified and susceptibility testing is performed if applicable.
Specimen Requirements
Blood
Container/Tube:
- Adult (>12 years of age) 1-Aerobic BacT Alert Blood Culture Bottle and 1 Anerobic BacT Alert Blood Culture Bottle. If specimen volume is insufficient to submit both aerobic and anaerobic bottles, 1 Aerobic BacT Alert Blood Culture Bottle is sufficient for testing.
- Infants/Children: 1 – Pediatric BacT Alert Blood Culture Bottle.
Specimen Volume:
- Adult: Optimal Volume: 10mL per bottle (Aerobic and Ananerobic) Minimum volume: 5mL per bottle (Aerobic and Anaerobic). If less than 10mL of blood is collected, place entire volume of collected blood into an Aerobic BacT Alert Blood Culture Bottle.
- Infants/Children: Optimal Volume: 5 mL Minimum volume: 2mL. Place entire collected volume into one Pediatric BacT Alert Blood Culture Bottle.
Specimen Transport Temperature
Ambient
Testing Algorithm
All recovered organisms are identified and susceptibiity testing is performed if applicable
Methodology
Automated or Manual Detection of Positive Blood Cultures with Conventional Identification Methods
Reference Values
No growth in 5 days
Test Classification and CPT Coding
87040
Additional charges and CPT codes may be added
Loinc Code
600-7
Specimen Collection Instructions
Collection Instructions: Collect specimen as follows:
- 1. Sterilize tops of bottles with 70% isopropyl alcohol.
- 2. Apply tourniquet to patient’s arm.
- 3. Palpate area to locate vein.
- 4. Thoroughly cleanse venipuncture site with ChloraPrep® applicator moving in concentric circles to periphery. Do not palpate vein after sterilizing area. If site must be touched during venipuncture, disinfect gloved finger. Allow to dry.
- 5. Using a syringe or butterfly collection kit for direct inoculation, draw blood into each BacT Alert® bottle.
Note:
- 1. Label bottles with patient’s name (first, last, and middle initial), medical record number (if appropriate), date of birth, date and time of specimen collection and collectors initials and/or ID number.
- 2.This test code is ordered for patients who have not received antibiotic therapy within the last 48 hours.
- 3. Every attempt should be made to obtain the requested volume for each blood culture ordered. Blood cultures will not be rejected for inadequate volume if visible blood is present in the blood culture botte.