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Test Code ANER Culture, Anaerobic

Performing Laboratory

YRMC, Microbiology

Day(s) Test Set Up

Monday through Sunday. 

Cultures are held for 5 days before a Final Report is issued.

Specimen Requirements

Submit only 1 of the following specimens - specimen source is required:

  • Abscess – Sterile, screw capped container, syringe with needle removed If the specimen is submitted in a syringe, the needle may be replaced with sterile syringe cap or sterile capped blunt cannula. Specimens submitted on culture swabs are sub-optimal for anaerobic culture.

     

    Volume: 1 – 2 ml aspirate material or abscess drainage

     

  • Body Fluid – Screw-capped, sterile container, evacuuated collection container. Specimens submitted on swabs are not acceptable.

     

    Volume: 5 ml of body fluid

     

  • Tissue – Screw-capped, sterile container(s) without fixative or preservative Specimen in formalin is not acceptable. Tissue specimens should not be placed into a culture swab prior to transport.

     

    Volume: 1 g of tissue if possible.

     

  • Trans-trachael Aspirate – Screw-capped, sterile container(s) or aspirate trap. If an aspirate trap specimen is submitted, attach the tubing firmly to the top of the aspirate trap to ensure specimen does not leak during transport.

     

    Volume: 1 ml minmum volume of trachael aspirate material

The following specimen sources are not suitable for anaerobic cultures and will not be accepted:

  • Throat (exception: Peritonsilar  Abscess)
  • Vagina
  • Cervix
  • Urethra
  • Urine (exception: Suprapubic Aspirate)
  • Sputum
  • Catheter tips
  • Mouth
  • Nasal
  • Eye
  • Ear

Specimen Transport Temperature

Ambient

Testing Algorithm

Anaerobic cultures are ordered in conjunction with an aerobic culture from the same source.

Methodology

Conventional culture methodologies

Reference Values

No anaerobes isolated.

Test Classification and CPT Coding

87075 - Culture, Anaerobic Convential

Loinc Code

635-3

Specimen Collection Instructions

Abscess
1.  For most open lesions and abscesses, remove the superficial flora by decontaminating the skin before collecting a specimen from the advancing margin or base. 

2.  Appropriate specimens include but are not limited to the following: Pleural Fluid, Pericardial Fluid, Peritoneal Fluid, Peritoneal Dialysate, Ascites and Synovial Fluid. Specimen Source is Required.
3.  A closed abscess is the specimen site of choice. Aspirate the abscess contents with a syringe and submit the specimen in the syring or expell the contents into a sterile, scre-capped container.
4.  Specimen source is required
Note: Label container with patient’s name (first, last, and middle initial), medical record number (if appropriate), date of birth, date and time of collection and collector’s initials an/or ID number.

 

Note:  Label container with patient’s name (first, last, and middle initial, medical record number (if appropriate), date of birth, date and time of collection and collector’s initials and/or ID number.

______________________________________________________________________________________

 

Body Fluid
 1. Collect aseptically. Disinfect site with alcohol if collecting by needle and syringe.
 2. Specimen source is required.
 

Note: Label container with patient’s name (first, last, and middle initial), medical record number (if appropriate), date of birth, date and time of collection and collector’s initials and/or ID number

______________________________________________________________________________________

 

Tissue
 1. Collect aseptically avoiding indegenous microbiota. Select caseous portion if available.
 2. Specimen source is required.
 

Note: Label container with patient’s name (first, last, and middle initial), medical record number (if appropriate), date of birth, date and time of collection and collector’s initials and/or ID number

______________________________________________________________________________________

 

Trans-trachael Aspirate
 1.Collect aseptically according to trans-trachael aspiration protocol. Trachael aspirates obtained by scution are not accpetable for anaerobic culture.
 2. Specimen source is required.


Note: Label container with patient’s name (first, last, and middle initial), medical record number (if appropriate), date of birth, date and time of collection and collector’s initials and/or ID number