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HelpTest Code AMISO Amylase, Isoenzymes, Serum
Necessary Information
Age and sex of patient are required.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Serum gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged and the serum aliquoted into a plastic vial within 2 hours of collection.
Useful For
Ruling out salivary amylase as the cause of elevated serum amylase
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| AMYSE | Amylase, Total, S | Yes, (Order AMS) | Yes |
| AMYPA | Amylase, Pancreatic, S | No | Yes |
| AMYSA | Amylase, Salivary, S | No | Yes |
Testing Algorithm
Total and pancreatic amylase are measured in the submitted serum specimen. Salivary amylase is calculated as the difference between the two measured results (salivary amylase = total amylase-pancreatic amylase).
Method Name
AMYSE, AMYPA: Colorimetric Rate Reaction
AMYSA: Calculation
Reporting Name
Amylase, Isoenzymes, SSpecimen Type
SerumSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 30 days | |
| Frozen | 30 days | ||
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | Reject |
Reference Values
AMYLASE, TOTAL
0-30 days: ≤6 U/L
31-182 days: 1-17 U/L
183-365 days: 6-44 U/L
1-3 years: 8-79 U/L
4-17 years: 21-110 U/L
≥18 years: 28-100 U/L
AMYLASE, PANCREATIC
0-<24 months: ≤20 U/L
2-<18 years: 9-35 U/L
≥18 years: 13-53 U/L
AMYLASE, SALIVARY
0-<18 years: Not established
≥18 years: ≤86 U/L
Day(s) Performed
Monday through Sunday
Report Available
1 to 3 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
82150 x 2
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| AMISO | Amylase, Isoenzymes, S | 24333-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| AMYPA | Amylase, Pancreatic, S | 1805-1 |
| AMYSA | Amylase, Salivary, S | 1809-3 |
| AMYSE | Amylase, Total, S | 1798-8 |
Forms
If not ordering electronically, complete, print, and send Gastroenterology and Hepatology Test Request (T728) with the specimen.